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human pulmonary artery endothelial cells hpaecs  (ATCC)


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    ATCC human pulmonary artery endothelial cells hpaecs
    Human Pulmonary Artery Endothelial Cells Hpaecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+artery+endothelial+cells/Primary+Pulmonary+Artery+Endothelial+Cells%3B+Normal%2C+Human/pmc13225285-69-15-25
    Average 99 stars, based on 103 article reviews
    human pulmonary artery endothelial cells hpaecs - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Direct red blood cell effect on thrombosis is dependent on the interaction of tissue factor and calcium with membrane phosphatidylserine
    Article Snippet: auma Acute Care Su ume 97, Number 1 rior literature has implicated red blood cells (RBCs) in the initiation of thrombosis and suggests that posttransfusion hypercoagulability may occur secondary to the effects of RBCs.. Elevated serum tissue factor is a known sequelae of acute trauma.. Phosphatidylserine (PS) is a prothrombotic phospholipid present within the RBC cell membrane.

    Article Title: Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites.
    Article Snippet: .. Human pulmonary artery endothelial cells (PAECs; ATCC PCS-100-022) were cultured according to the supplier’s instructions. ..

    Article Title: In vivo gene editing of human hematopoietic stem and progenitor cells using envelope-engineered virus-like particles.
    Article Snippet: Vladimir V. Botchkarev Jr., Sean Harrington, Matteo Stoppato, Alexander Justen, Casey Kimber, Anjali Kapuria, Keylie M. Gibson, Chi-Shuen Chu, Yuanxin Xu, Kelsey Haugh , Ramya Ankala, Nathan Kipniss , Andre DeGroot , Emerson Moore, Rowena de Jesus, Funmi Adewale , Kathy Daniels, Samantha Crocker, Anna Liang, Shannon Joyce, Nicole Roberto , Pamela Angel, Derek Smith, Athena Wong, Nick Adler, Valeria Berlfein, Shirisha Amatya, Patricia Cruite, Shariq Usmani, Albert Ruzo, Benjamin Ferland, Sundeep Chandra, Edward J. Rebar, Jagesh V. Shah, Kyle Trudeau & Luca Biasco

    Article Title: Extracellular vesicles mediate the communication between multiple myeloma and bone marrow microenvironment in a NOTCH dependent way
    Article Snippet: HMCL were cultured in RPMI1640 (Euroclone, Italy) supplemented with 10% Fetal bovine serum (FBS) (Euroclone, Italy), 100U/ml penicillin/streptomycin (Microgem, Italy) and 2mM L-glutamine (Microgem, Italy). .. The primary human pulmonary artery endothelial cells (HPAEC, ATCC® PCS-100-022) were purchased from the American Type Culture Collection and cultured in Vascular Basal medium (ATCC® PCS-100-030TM) supplemented with Endothelial cells Growth Kit-VEGF (ATCC® PCS100-041TM) following the manufacturer instruction. .. Human embryonic kidney 293 cells, HEK293T (ATCC® CRL-157), HeLa cells (ATCC® CCL-2TM), and the murine pre-osteoclasts Raw264.7 (ATCC® TIB-71) were purchased from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM; Euroclone, Italy) with 10% FBS, 100U/ml penicillin/streptomycin and 2mM L-glutamine.

    In Vitro:

    Article Title: Inhaled seralutinib exhibits potent efficacy in models of pulmonary arterial hypertension
    Article Snippet: .. Human cell lines were obtained from commercial vendors and propagated under recommended culture conditions in a humidified incubator at 37°C, with 5% CO2 in air: NCI-H1703 (ATCC, Manassas, VA, USA), Human Pulmonary Arterial Smooth Muscle Cells (HPASMC, Cell Applications, San Diego, CA, USA), Human Lung Fibroblasts (HLF; Cell Applications, San Diego, CA, USA), HEK-293T (ATCC), and Human Pulmonary Artery Endothelial Cells (HPAECs, Cell Applications, San Diego, CA, USA) In Vitro Characterization of Compounds Compound activity was assessed in platelet-derived growth factor receptor alpha (PDGFRα), PDGFR beta (PDGFRβ) and colony stimulating factor 1 receptor (CSF1R) enzymatic assays at Carna Biosciences Inc. (Kobe, Japan). .. A c-KIT enzymatic assay was performed at Gossamer Bio (San Diego, CA, USA) using the Promega c-KIT Kinase Enzyme System (Promega Corporation, Madison, WI, USA).

    Activity Assay:

    Article Title: Inhaled seralutinib exhibits potent efficacy in models of pulmonary arterial hypertension
    Article Snippet: .. Human cell lines were obtained from commercial vendors and propagated under recommended culture conditions in a humidified incubator at 37°C, with 5% CO2 in air: NCI-H1703 (ATCC, Manassas, VA, USA), Human Pulmonary Arterial Smooth Muscle Cells (HPASMC, Cell Applications, San Diego, CA, USA), Human Lung Fibroblasts (HLF; Cell Applications, San Diego, CA, USA), HEK-293T (ATCC), and Human Pulmonary Artery Endothelial Cells (HPAECs, Cell Applications, San Diego, CA, USA) In Vitro Characterization of Compounds Compound activity was assessed in platelet-derived growth factor receptor alpha (PDGFRα), PDGFR beta (PDGFRβ) and colony stimulating factor 1 receptor (CSF1R) enzymatic assays at Carna Biosciences Inc. (Kobe, Japan). .. A c-KIT enzymatic assay was performed at Gossamer Bio (San Diego, CA, USA) using the Promega c-KIT Kinase Enzyme System (Promega Corporation, Madison, WI, USA).



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    In vivo activin A inhibition improves pulmonary hypertension (PH) phenotype comparably or more than ET-1 (endothelin-1) blockade. A , Experimental design: wild-type (WT) and VE-cadherin (vascular endothelial cadherin)–INHBA (inhibin β-A)-Tg (TG/transgenic) mice were exposed to hypoxia (10% O 2 ) for 3 weeks, with vehicle (VEH), FST (follistatin; 8.5 μg/kg), bosentan (BOS; 30 mg/kg), or FST+BOS administered during the final 2 weeks. B , Right ventricular systolic pressure (RVSP; n=4–9). C , Fulton index (RV/[LV+S] [right ventricle to left ventricle plus septum] ratio; n=4–8). D , Representative hematoxylin and eosin–stained lung sections. Blue arrows indicate vessels. E , Representative immunofluorescent staining of α-SMA (α-smooth muscle actin protein; green, SMC [smooth muscle cell] marker), vWF (von Willebrand Factor; red, endothelial cell [EC] marker), and DAPI (4′,6-diamidino-2-phenylindole; blue, nuclei). White arrows indicate vessels. F , Quantification of pulmonary artery muscularization (non-, partial-, full; n=12–15 fields from 3–4 mice). PA indicates pulmonary artery. G , Lung ET-1 mRNA expression (n=3–4). H , mRNA expression of INHBA, ET-1, eNOS (endothelial NO synthase), SOD2 (superoxide dismutase 2), fibronectin, SLUG (snail family transcriptional repressor 2), CD31 (cluster of differentiation 31), and BMP4 (bone morphogenetic protein 4) in lung ECs isolated from WT and TG mice (n=3–4). Data are mean±SEM. P <0.05 is deemed statistically significant. Tests: 1-way ANOVA with Tukey post hoc test for B , C , and G ; 2-way ANOVA with Tukey post hoc test for F ; 2-sided Student t test for H .

    Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

    Article Title: Activin A–Endothelin-1 Axis Governs Pulmonary Vascular Remodeling: Mechanistic Basis for Emerging Therapies in PAH

    doi: 10.1161/ATVBAHA.125.323681

    Figure Lengend Snippet: In vivo activin A inhibition improves pulmonary hypertension (PH) phenotype comparably or more than ET-1 (endothelin-1) blockade. A , Experimental design: wild-type (WT) and VE-cadherin (vascular endothelial cadherin)–INHBA (inhibin β-A)-Tg (TG/transgenic) mice were exposed to hypoxia (10% O 2 ) for 3 weeks, with vehicle (VEH), FST (follistatin; 8.5 μg/kg), bosentan (BOS; 30 mg/kg), or FST+BOS administered during the final 2 weeks. B , Right ventricular systolic pressure (RVSP; n=4–9). C , Fulton index (RV/[LV+S] [right ventricle to left ventricle plus septum] ratio; n=4–8). D , Representative hematoxylin and eosin–stained lung sections. Blue arrows indicate vessels. E , Representative immunofluorescent staining of α-SMA (α-smooth muscle actin protein; green, SMC [smooth muscle cell] marker), vWF (von Willebrand Factor; red, endothelial cell [EC] marker), and DAPI (4′,6-diamidino-2-phenylindole; blue, nuclei). White arrows indicate vessels. F , Quantification of pulmonary artery muscularization (non-, partial-, full; n=12–15 fields from 3–4 mice). PA indicates pulmonary artery. G , Lung ET-1 mRNA expression (n=3–4). H , mRNA expression of INHBA, ET-1, eNOS (endothelial NO synthase), SOD2 (superoxide dismutase 2), fibronectin, SLUG (snail family transcriptional repressor 2), CD31 (cluster of differentiation 31), and BMP4 (bone morphogenetic protein 4) in lung ECs isolated from WT and TG mice (n=3–4). Data are mean±SEM. P <0.05 is deemed statistically significant. Tests: 1-way ANOVA with Tukey post hoc test for B , C , and G ; 2-way ANOVA with Tukey post hoc test for F ; 2-sided Student t test for H .

    Article Snippet: Human pulmonary artery ECs (PAECs; no. C-12241; PromoCell) and pulmonary artery smooth muscle cells (PASMCs; no. C-12521; PromoCell) were cultured in HuMedia-EG2 (Kurabo) and smooth muscle cells growth medium 2 (PromoCell), respectively, at 37 °C and 5% CO 2 .

    Techniques: In Vivo, Inhibition, Transgenic Assay, Staining, Marker, Expressing, Isolation